ProbeBuilder-GPT: Design Next-Generation Protein Tags
Your design assistant for engineering protein and antibody tags for cryo-ET and spatial multi-omics, inspired by Sanjay Srivatsan.
Access ProbeBuilder-GPTWhat is ProbeBuilder-GPT?
ProbeBuilder-GPT is an AI-powered design assistant for engineering innovative protein or antibody tags that report spatial or ultrastructural information. It specializes in creating tools like engineered enzymes (e.g., peroxidases for EM tagging) or split-fluorophores for spatial multi-omics, guiding researchers through the entire design-build-test cycle for novel tagging strategies.
Engineer Probes for Cellular Cartography
Expert Tag Design Advice
Advises on tag design: where to insert a tag in a protein for exposure and functionality, optimal linker lengths, and validation assays (Western blot, imaging).
Understand Method Constraints
Explains cryo-ET sample prep constraints (maintaining native structure, tag size limits) and considerations for spatial multi-omics applications.
Predict Impact & Automate Design
Predicts if tag fusion will disrupt protein structure. Outputs primer sequences (FASTA/text) for cloning tags at specific sites.
Plan Validation Experiments
Helps plan high-throughput validation experiments, such as generating CSV plate maps for testing multiple tag variants in parallel.
Structured Design Outputs
Provides design rationale in paragraphs, sequences in Markdown code blocks, and design options/validation steps in bullet points or tables.
Confidential & IP Conscious
Redacts proprietary sequences by default, keeps experimental imaging in-session, and reminds users of material transfer or licensing for patented tags.
Ready to Build Your Next Advanced Probe?
Collaborate with ProbeBuilder-GPT to design and validate cutting-edge protein tags for high-resolution spatial and ultrastructural studies.
Start Designing Probes