NeuroScreen-GPT: End-to-End In Vivo CRISPR Screen Design
Your guide for pooled in vivo CRISPR screens tracked by whole-brain imaging, inspired by Will Allen. From gRNA libraries to brain activity maps.
Access NeuroScreen-GPTWhat is NeuroScreen-GPT?
NeuroScreen-GPT is an end-to-end guide for designing and interpreting pooled in vivo CRISPR screens coupled with whole-brain imaging techniques like light-sheet or light-field microscopy. It assists researchers in navigating the complexities of gRNA library selection, viral vector delivery, imaging schedules, and linking genetic perturbations to observed brain-wide activity or structural phenotypes.
Master Your Whole-Brain CRISPR Screens
Comprehensive Screen Design
Guides choices for gRNA libraries (targeting specific genes/pathways), viral vectors, and optimal imaging schedules for your in vivo screens.
Interpret Brain Activity Maps
Helps interpret how specific gene perturbations might cause observed changes in brain activity maps and suggests statistical methods to link genotype to phenotype.
Automated gRNA & Image Analysis
Designs balanced gRNA pools, aligns 3D brain images to atlases (e.g., Allen Brain Atlas), and merges sequencing with imaging data to find candidate genes.
Clear & Actionable Outputs
Provides Markdown summaries, CSV tables for "hit" genes (with metrics like activity change, p-value), and JSON with spatial coordinates for visualization.
Expert & Engaging Dialogue
Bridges molecular techniques and systems neuroscience with an enthusiastic and highly informed tone, making complex topics accessible.
Secure & Ethical Guidance
Keeps animal/lab identifiers internal, anonymizes human data, and reminds users of IACUC guidelines for animal experiments.
Ready to Illuminate Brain-Wide Genetic Effects?
Partner with NeuroScreen-GPT to design and execute groundbreaking in vivo CRISPR screens with whole-brain imaging readouts.
Plan Your NeuroScreen