Membrane-Design-GPT: AI-Powered Transmembrane Protein Engineering
Your AI protein design coach for creating stable transmembrane protein scaffolds using co-evolutionary data and diffusion generative models, inspired by Sergey Ovchinnikov.
Access Membrane-Design-GPTWhat is Membrane-Design-GPT?
Membrane-Design-GPT is an AI-driven protein design coach specializing in the creation of stable and functional transmembrane protein scaffolds. It leverages co-evolutionary data and diffusion generative models to help researchers design novel membrane proteins, explaining key principles like hydrophobic matching and common structural motifs, and guiding them through the design-build-test cycle.
Engineer Novel Membrane Proteins with AI Precision
Expert Design Guidance
Explains factors like hydrophobic thickness matching the membrane and common gating motifs. Proposes sequence scaffolds or mutations to improve stability or function.
Guide Expression & Validation
Suggests optimal expression systems (e.g., E. coli vs Pichia) for membrane proteins and how to validate designs (circular dichroism, functional assays).
AI-Generated Protein Candidates
Runs latent diffusion models conditioned on membrane topology to generate candidate protein sequences, incorporating co-evolutionary restraints for realistic folding.
Predict Structure & Stability
Validates top designs by predicting structures with AlphaFold Multimer or RosettaMP, and estimates membrane insertion free energies (ΔG_mem).
Lab-Ready Design Outputs
Provides design rationale (e.g., “Design A: four-helix bundle”), FASTA sequences, CSV for cloning orders, and JSON for automated pipelines.
Secure & Responsible Design
Flags designs similar to known toxins or viral proteins for biosecurity. Requires user confirmation for saving proprietary designs and deletes intermediate data.
Ready to Engineer Novel Membrane Proteins?
Collaborate with Membrane-Design-GPT to leverage AI, co-evolutionary data, and diffusion models for creating the next generation of transmembrane proteins.
Design Your Membrane Protein