LineageGraph-GPT: Trace Developmental Histories with CRISPR
Your lineage-tracing assistant for turning homing-CRISPR barcode data into developmental trees and 3D fate maps, inspired by Reza Kalhor.
Access LineageGraph-GPTWhat is LineageGraph-GPT?
LineageGraph-GPT is a specialized assistant for lineage tracing, designed to transform raw homing-CRISPR barcode data into insightful developmental trees and 3D fate maps. It helps researchers reconstruct cell lineage histories from CRISPR recording systems by guiding them through data parsing, error correction, phylogenetic tree building, and interpretation of complex lineage information.
Reconstruct Cellular Ancestries with Precision
Understand Lineage Concepts
Explains how CRISPR barcodes mutate over time to record lineage, guides barcode sequence parsing, error correction, and phylogenetic tree building.
Interpret Lineage Data
Highlights confidence levels in tree branches, identifies potential noise, and helps users make sense of complex lineage relationships.
Automated Barcode Analysis
Parses FASTQ files of CRISPR barcode reads, clusters similar barcodes to account for sequencing errors, and builds lineage trees using phylogenetic methods.
Estimate Noise & Confidence
Estimates recording noise (false edits, missed edits) and provides confidence scores for each lineage split in the reconstructed tree.
Flexible Data Export
Exports results as Newick strings for trees, or 3D models (e.g., .gltf, JSON with coordinates) for spatial visualization of lineage.
Confidential Lineage Analysis
Keeps embryo images and experimental details local, respects confidentiality for unpublished data, and purges raw sequence data post-analysis.
Ready to Map the Tree of Life Within Your Samples?
Let LineageGraph-GPT help you decode complex developmental histories and visualize cellular relationships with cutting-edge CRISPR recording analysis.
Explore Lineage Tracing