GeneWriter-GPT: Engineer Large DNA Insertions
Your assistant for designing pegRNAs and RT templates for gene writing systems (PASTE/TOME), predicting efficiencies, and automating primer orders, inspired by Omar Abudayyeh & Jonathan Gootenberg.
Access GeneWriter-GPTWhat is GeneWriter-GPT?
GeneWriter-GPT is a specialized AI assistant for large DNA insertion technologies, focusing on the design and implementation of novel gene-writing systems like PASTE or TOME. It helps researchers create pegRNAs and reverse transcriptase (RT) templates, predict editing efficiencies, troubleshoot experimental challenges, and automate the generation of constructs for genome engineering.
Advance Your Gene Writing Capabilities
Explain Gene Writing Systems
Clearly explains how reverse transcriptase fusions (e.g., Cas9-RT in PASTE systems) work to precisely insert large DNA sequences into the genome.
Design pegRNAs & RT Templates
Designs pegRNAs (prime editing guide RNAs) and RT templates for desired insertions, suggesting safe harbor loci and optimizing for predicted efficiency.
Debug & Validate Insertions
Helps debug issues like unexpected indels or low integration efficiency. Outlines steps to validate successful insertions in cells or animals (PCR, sequencing).
Predict pegRNA Performance
Simulates pegRNA secondary structure and RT template binding (checking ΔG, avoiding hairpins). Ranks designs by predicted efficiency.
Formatted Design Sheets
Provides "Guide Design Sheets" (Markdown), insert sequences (FASTA), and CSV/lists of oligonucleotides for constructing vectors.
Secure & Ethical Engineering
Flags potentially harmful insertions (gene drives, pathogenic sequences) and ensures appropriate containment/approval considerations. Deletes provided sequences.
Ready to Write the Next Chapter in Genome Engineering?
Collaborate with GeneWriter-GPT to design and implement cutting-edge large DNA insertion strategies for your research and therapeutic applications.
Design Your Gene Writer