DxBuilder-GPT: Engineer Next-Gen CRISPR Diagnostics
Your diagnostic design assistant for automating CRISPR-based assay design (INSPECTR/SHERLOCK), reaction simulations, and regulatory documentation, inspired by Jim Collins.
Access DxBuilder-GPTWhat is DxBuilder-GPT?
DxBuilder-GPT is a diagnostic design assistant that automates and streamlines the creation of CRISPR-based assays like INSPECTR and SHERLOCK. It helps researchers and clinicians design guides and primers, simulate reactions, formulate freeze-dried reagents, and even draft regulatory documentation for paper-strip tests, covering both scientific and practical deployment aspects.
Accelerate Your Diagnostic Development Pipeline
Understand CRISPR Dx Chemistry
Explains the chemistry of CRISPR diagnostics (e.g., how Cas13 detection works) to newcomers and advises on multiplexing or lyophilization tweaks.
Automated Assay Design
Fetches pathogen genomes and designs RPA/LAMP primers and Cas13 guide RNAs for target sequences, ensuring no interference for multiplex targets.
Simulate & Predict Performance
Simulates amplification and detection kinetics using tools like NUPACK to estimate limit of detection (LOD) and outputs QC spreadsheets.
Regulatory Document Drafting
Outlines steps for regulatory submissions (EUA or 510(k) filings) and can draft sections of regulatory documents like test protocols or validation summaries.
Structured Protocols & Data
Provides Markdown protocols summarizing assay steps, CSV tables of primer/guide sequences, and clear considerations (e.g., "Include RNase H step").
Secure & Responsible Design
Removes patient identifiers, warns if primers hit select agent sequences, and deletes sensitive sequence data after design completion.
Ready to Build Rapid, Reliable Diagnostics?
Partner with DxBuilder-GPT to design and deploy cutting-edge CRISPR-based diagnostic assays with speed and precision.
Develop Your Diagnostic Assay