CasDesigner-GPT: Engineer Next-Generation CRISPR Enzymes
Your protein engineering assistant for designing CRISPR-Cas variants with expanded PAM recognition or improved specificity, inspired by Pranam Chatterjee.
Access CasDesigner-GPTWhat is CasDesigner-GPT?
CasDesigner-GPT is a protein engineering assistant specializing in the design of CRISPR-Cas variants. It helps researchers create enzymes with expanded Protospacer Adjacent Motif (PAM) recognition or improved specificity by explaining Cas9-PAM interactions, guiding mutation site selection, suggesting validation assays, and automating aspects of the design process.
Revolutionize Your CRISPR Engineering
Understand PAM Interactions
Explains how Cas9 residues interact with PAM sequences and how specific mutations can alter PAM preference (e.g., to recognize NGN instead of NGG).
Guided Mutation & Cloning
Walks users through selecting mutation sites to broaden PAM range and provides primer designs for cloning via Golden Gate or site-directed mutagenesis.
Validation Assay Strategy
Suggests assays to validate the new enzyme’s on-target activity and off-target profile (e.g., in vitro cleavage, targeted sequencing).
AI-Powered Mutation Design
Proposes mutations to expand PAM tolerance using ML models or known mutational data. Optionally runs AlphaFold for structural integrity checks.
Ready-to-Use Sequences
Outputs lists of mutations, degenerate oligos for library creation, and mutant amino acid sequences in FASTA format, all clearly rationalized.
Responsible Design Practices
Highlights potential dual-use applications (e.g., gene drives) and ensures users are aware of containment and biosafety guidelines.
Ready to Engineer Custom Cas Enzymes?
Collaborate with CasDesigner-GPT to design and validate novel CRISPR-Cas variants with tailored PAM recognition and specificity for your research needs.
Start Your Cas Design Project